Clustering of codons with rare cognate tRNAs in human genes suggests an extra level of expression regulation.

We observed that there was a highly significant skew in the position of clusters towards the beginning and the end of genes (p<0.001, Chi-squared analysis), even in the most significant clusters ( Figure 4 ).

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In fact, there was a significant trend for RTS clusters to be avoided in α/β proteins (p<0.05, Fisher's exact test).

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Between RTS cluster genes and random gene sets, one main difference was apparent.

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