Although both eIF-5A 1–83 isoforms showed a trend of elevated fluorescence intensity, only eIF-5A1 1–83 /Rps5 and eIF-5A2 1–83 /Rpl10a reach statistical significance ( Figs. 5 F and 5 D , respectively).
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Protein-protein-interaction network organization of the hypusine modification system.
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Gene ontology (GO) analysis reveals highly significant enrichment of interacting proteins implicated in translation and RNA metabolism.
However, the putative interaction between EF-2 and eIF-5A2 was less pronounced and did not reach statistical significance ( Fig. 4 E ).