However, when restricted to the sites included in this study, the interface sites tended to be slightly more conserved than non-interface sites but the difference did not reach statistical significance (Wilcoxon rank sum test p = 0.072) ( Table S3 ).
2
0.0720
0.0720
The sentences
Three mutants: R154K, T110N, and L6I, also showed a trend of reduced fitness, although not significantly different from the prototype (95% CI p>0.05).