Barrier disrupting effects of alternaria alternata extract on bronchial epithelium from asthmatic donors.

At 24 h post-challenge, while inter-treatment differences approached significance, the significance threshold was not crossed.

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Clad100 did not increase basolateral IL-8 release above baseline and heat treatment of the Alternaria extract (Alt100HT) tended to reduce the stimulation of basolateral IL-8 release, but this failed to reach statistical significance.

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This may be significant in terms of establishing appropriate concentration gradients for chemoattractants that direct cells to the luminal or subepithelial compartments.

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