CD40L treated DCs were similar to untreated DCs, while CpG, TNF-α and LPS matured DCs showed a trend towards increased acidification.
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Intracellular regulation of cross-presentation during dendritic cell maturation.
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DCs given a combination of TLR ligand and OVA beads cross-presented significantly less well than the same DCs (UT or CpG matured) incubated with OVA beads alone; for LPS matured DCs, the decrease in cross-presentation after extra addition of LPS during bead incubation did not reach statistical significance ( Figure 3A ).