Adipose tissue NAPE-PLD controls fat mass development by altering the browning process and gut microbiota.

Nevertheless, we cannot exclude that insulin resistance could affect those organs at a later time since phosphorylation of IRβ is also significantly affected in skeletal muscle and there is an important trend towards reduced insulin-induced phosphorylation of IRβ in adipose tissue.

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Interestingly we found that after 24 h incubation in culture medium, adipose tissue explants from cKO mice tend to reproduce the in vivo phenotype on browning markers, although this decrease did not reach statistical significance ( Supplementary Fig. 6 ).

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