Puberty-specific promotion of mammary tumorigenesis by a high animal fat diet.

Although the trends of increased incidence and decreased latency of the HFD-LFD group did not reach statistical significance compared with the LFD-HFD group (Fig. 1d ), the overall pattern was similar to continuous HFD vs. continuous LFD (Fig. 1e ).

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Ctnnb1 (β-catenin) expression was also increased in pretumor mammary glands of HFD-LFD mice and showed a trend toward increased expression in continuous HFD mice, indicating another early effect of peripubertal exposure to HFD.

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