The expression levels of PLOD1 was significantly increased in both AF-S and AF-nS clones under TGFβ3 conditions; all three PLOD genes showed a trend toward higher expression in AF-S clones ( Fig 5 , middle left panel).
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Novel Immortal Cell Lines Support Cellular Heterogeneity in the Human Annulus Fibrosus.
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Within 4 and 6 days of culturing, a clear trend towards significantly different COL1A1/ProCOL1A1 ratios between AF-S and AF-nS became apparent ( Fig 4C ).