Enforced Expression of Hoxa3 Inhibits Classical and Promotes Alternative Activation of Macrophages In Vitro and In Vivo.

Interestingly Hoxa3 increased total Stat6 protein accumulation in the unstimulated cells ( Fig 7C ), although no detectable transcript difference was apparent ( Fig. 7A , 7B ), suggesting that Hoxa3 may also mediate stabilization of Stat6 protein.

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Protein transduction of Hoxa3 mCh showed a trend of increased mean fluorescence intensity (MFI) of cell surface F4/80 protein in non–db-derived mφs (Hoxa3 mCh MFI = 27725.5 ± 13624 versus mCherry MFI = 17380.8 ± 13690, p = 0.2) and in the db-derived population (Hoxa3 mCh MFI = 11746 ± 7681 versus mCherry MFI 8913 ± 5499, p = 0.1).

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