<i>Ex vivo</i> response to mucosal bacteria and muramyl dipeptide in inflammatory bowel disease.

After stimulation with MDP in the presence of LB, even though the differences are not significant, there is a clear trend of increased secretion of IL2, IL6, IL8, IL17 and IFNγ in UC, while no change was observed in CD.

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In contrast, in the presence of LB UC showed an increasing trend of spontaneous IL8 production, while MDP stimulation resulted in lower IL8 production in the presence of antibiotics.

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In contrast, samples from UC showed a trend of increase of cytokines such as IL6 and IL8 when stimulated with MDP in the presence of LB.

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The addition of antibiotics to the culture produced only a slight trend towards increased spontaneous cytokine secretion, both in CD and in UC, but no difference was significant, except for increased IL8 secretion in UC.

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