Organ culture storage of pre-prepared corneal donor material for Descemet's membrane endothelial keratoplasty.

At day 2, the area of the wound not covered by the migrating cells was smaller in the En-OC group than in the standard organ culture group (39±7% vs 31±11%, n=3), but this failed to reach statistical significance.

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Use of En-OC showed a trend towards increased cell density in the wounded area but it does not increase cell proliferation, as detected by Ki-67 staining, suggesting that the effect of growth factor supplementation is to primarily increase cell migration.

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