An observed trend ( p between 0.05 and 0.1) is indicated with “#” and the p value stated in the figures. qRT PCR quantification Relative quantification is based on internal reference genes to determine virtual mRNA levels of target genes.
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Zinc deficiency and low enterocyte zinc transporter expression in human patients with autism related mutations in SHANK3.
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The sentences
In addition, a reduction of ZIP4 protein in samples from PMDS patients was observed as a clear trend.
On protein level, for ZIP4, a significant loss of transporters at the membrane occurs and the analysis of cell protein homogenate reveals a strong trend towards a decrease in total ZIP4 and a significant decrease in ZIP2.