Similarly, the model predicts that loading efficiency (%) significantly increases with increasing kafirin quantities (mg), while the effect of prednisolone quantities (mg) on loading efficiency demonstrated a strong trend at the quadratic level ( p = 0.0522) ( Figure 2 , Equation (4) and Table 3 ).
Prednisolone loading (%) significantly increased with increasing quantities of prednisolone, while there was a negative trend with kafirin quantity ( Figure 1 , Equation (3)); though this was not statistically significant ( Table 3 ).
The starting concentration of kafirin was the only significant term described in the quadratic model of loading efficiency, but only demonstrated a trend towards significance.