Leucine elicits myotube hypertrophy and enhances maximal contractile force in tissue engineered skeletal muscle in vitro.

Phosphorylation of 4EBP‐1 Thr37/46 , a regulator of cap‐dependent translation appeared to increase in response to leucine, although this effect did not reach statistical significance ( p = 0.07, Figure 1 a).

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Five days of leucine supplementation at increasing doses had no impact on Trim63 ( p = 0.88) or Fbxo32 ( p = 0.47) mRNA expression, or the levels of Gabarap ( p = 0.88) or Map1lc3a ( p = 0.07), although, the latter approached significance through the observed increase in expression seen with 20 mM leucine supplementation. 3.2.

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