Genomic upregulation of cardiac Cav1.2α and NCX1 by estrogen in women.

Cav1.2α and NCX1 showed a tendency to be more abundant on the epicardium compared to the endocardium, but these differences did not reach statistical significance in any of the groups (Fig. 3 ).

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Male human iPS-CMs treated with 17-β-estradiol showed a trend for increased peak current density at − 10 mV as compared to vehicle-treated iPS-CMs (17-β-estradiol-treated − 4.49 ± 0.50 pA/pF, n = 5 vs. vehicle-treated − 3.19 ± 0.88, n = 5, NS at P < 0.05) (Fig. 5 a).

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