Although both the IFNγ ELISA and ICS assays showed a significant trend towards improved inter-site comparability at higher measured responses, the effect was stronger in the former assay where inter-site CVs were below 10% at the highest measurable responses.
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Assay optimisation and technology transfer for multi-site immuno-monitoring in vaccine trials.
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The sentences
There was a trend towards improved comparability post-training for both CD4+ T cell and CD8+ T cell ICS assays but this did not reach statistical significance.