Adherent vs. Free-Floating Neural Induction by Dual SMAD Inhibition for Neurosphere Cultures Derived from Human Induced Pluripotent Stem Cells.

Relative numbers of β-III-tubulin-positive cells showed a trend toward higher values for the adherent induction protocol without reaching significant levels (Figure 3g ).

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The cell lines induced via EBs contained a higher density of Nestin-positive NSCs (significantly at passage 5) and after differentiation resulted in a higher density of total cells and a trend toward more MAP2-positive neurons (nominally significant at passage 2).

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