Downregulation of p53 drives autophagy during human trophoblast differentiation.

7 a, b), LC3B-II levels showed a trend to increase in presence of leupeptin, which, however, did not reach statistical significance, when compared to control (Fig. 7 a, c).

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Consistent with the treatment applied, forskolin was identified as highly significant activating regulator.

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Interestingly, leupeptin per se increased low LC3B-II levels in vehicle control cells by 63%, which did not reach statistical significance, but suggested some extent of basal autophagic activity in non-stimulated BeWo cells (Fig. 4 a, b).

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