Cortical bone volume and cortical thickness also showed a trend towards increasing in male Npffr2 − /− mice (Fig. 6i,j ).
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Diet-induced adaptive thermogenesis requires neuropeptide FF receptor-2 signalling.
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Thyrotropin-releasing hormone ( Trh ) mRNA showed a strong trend towards increasing in WT but not Npffr2 − /− mice in response to HFD (Supplementary Fig. 6c ).