( B ) Consistent with RNAseq data, second harmonic imaging analysis of end-of-study tumors revealed that anti-MMP9–treated tumors showed a trend for decreased fibrillar collagen content as compared to control IgG–treated tumors.
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MMP-9 inhibition promotes anti-tumor immunity through disruption of biochemical and physical barriers to T-cell trafficking to tumors.
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Fibrillar-collagen intensity quantification across entire images confirmed a trend toward decreased fibrillar collagen present in the anti–MMP-9–treated tumors, although this did not reach statistical significance.