Based on RT-qPCR, our data showed a trend towards increased vegfaa expression at 1 dpci, although this was not statistically significant, unlike the concomitant changes in nrp1 .
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Neuropilin 1 mediates epicardial activation and revascularization in the regenerating zebrafish heart.
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From 3 dpci and later, immunoblotting revealed an upregulation of Nrp1 proteins in the injured hearts compared with sham-operated hearts, although this did not reach statistical significance ( Fig. 1 B).