Expression of the E1α subunit of BCKD showed a significant trend to increase on all days of differentiation, where D4 and D5 were significantly higher compared to D1 of differentiation ( p < .05) (Figure 2 b).
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Depletion of branched-chain aminotransferase 2 (BCAT2) enzyme impairs myoblast survival and myotube formation.
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Interestingly, in cells depleted of BCAT2, phosphorylation of S6 was significantly reduced at D2 ( p < .05) and showed a trend to be reduced at D3 and D4 as well (Figure 3 b and g).