Moreover, from the IS compounds that eluted at the beginning of the chromatogram, L-carnitine-D 3 showed a trend to decrease the signal and such trend was not observed in isoleucine 13 C, 15 N.
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Troubleshooting in Large-Scale LC-ToF-MS Metabolomics Analysis: Solving Complex Issues in Big Cohorts.
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Regarding the normalization by IS mix, TUS plots for both modes showed a clear trend to increase the signal along the injection order.