Comprehensive analysis of tumor necrosis factor receptor TNFRSF9 (4-1BB) DNA methylation with regard to molecular and clinicopathological features, immune infiltrates, and response prediction to immunotherapy in melanoma.

6 ), which, however, did not reach statistical significance ( P = 0.17, Wilcoxon Mann–Whitney U ). 4.

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1 ), which had shown highly significant correlations between methylation and lymphocyte score in the TCGA cohort.

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Furthermore, we found methylation to be significantly correlated with PFS and a marked trend towards higher mean methylation levels in non-responding tumors in comparison to tumors responding to anti-PD-1 therapy (UHB ICB cohort).

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