Furthermore, when controlling for metastasis at the time of diagnosis, clustering using the 22-miRNA profile (reduced to 18 miRNAs due to mapping attrition) showed a trend toward significance (median RFS 8 vs 27 months, and 64 vs 105 months for metastatic and non-metastatic subgroups, respectively, stratified log rank p = 0.11).
Analysis using the supervised signed average expression method produced similar results with a strong trend for survival (Fig.
For transcription factor binding site analysis, there is still significant uncertainty about experimentally verified annotations, and a lot of nominally significant sequences were identified, so an equally stringent significance cut off of FDR <0.05 was used in both 22-miRNA and 5-miRNA restrictive gene target lists.