Although no significant effect of TRH stimulation on equine ACTH stability was detected in our population (Table 2 ), the P value for an effect of TRH stimulation was close to the level of significance utilized (.05) in the study.
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The effect of freeze-thaw cycles on determination of immunoreactive plasma adrenocorticotrophic hormone concentrations in horses.
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The sentences
Because the effect of TRH stimulation did not reach statistical significance, data from groups 1 and 2 and groups 3 and 4 were combined to obtain a control group and a PPID group, respectively, which increased the power of the study (1 – β = 0.65 overall).