Differential effects of stress exposure via two types of restraint apparatuses on behavior and plasma corticosterone level in inbred male BALB/cAJcl mice.

Tube‐restraint–stressed mice traveled longer distances than control mice in the first and second 5‐minute blocks of the test ( P < .0001 and P = .0036, respectively), and film‐restraint stress mice also exhibited longer distances traveled than control mice in the first block, although the result failed to reach significance after Bonferroni correction ( P = .0306).

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The statistical analysis revealed that there were nominally significant main effects of stress on the distance traveled in the dark chamber (Figure 2 B, left: F 2,66 = 3.40, P = .0394) and time spent in the light chamber (Figure S1 A: F 2,66 = 3.71, P = .0297).

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The post hoc comparisons revealed that film‐restraint–stressed mice exhibited less immobility than control mice ( P = .0026) and showed a trend toward decreased immobility compared with tube‐restraint–stressed mice, although this result did not reach significance after Bonferroni correction ( P = .0177).

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