An analytical study of neocartilage from microtia and otoplasty surgical remnants: A possible application for BMP7 in microtia development and regeneration.

BMP7-treated cell/scaffold constructs showed a trend toward increasing width and length compared to their non-treated counterparts ( Fig 2 and Table 2 ).

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However, microtia cells treated with BMP7 showed a strong trend toward increased expression of type II collagen and elastin when compared to all other groups.

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Microtia samples with BMP7 addition showed a trend toward greater expression of elastin, type II collagen and SOX5 compared to all other groups ( Table 3 , trendline slopes), and both BMP7-enhanced groups exhibited a decreasing trend in type III collagen gene expression when compared with their respective groups without the growth factor ( Table 3 , trendline slopes).

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Rectangles highlight those clusters with a highly significant p-value (0.05).

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RT-qPCR analysis showed an increasing trend in gene expression levels of elastin, type II collagen and SOX5 in the microtia group in the presence of BMP7 compared to the other three groups, and both microtia and normal neocartilage groups with BMP7 supplementation were found with decreased type III collagen expression compared to the respective two groups without BMP7 addition.

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