There is no magic bullet: the importance of testing reference gene stability in RT-qPCR experiments across multiple closely related species.

PeerJ · 2020 · PMC7409783 · PMID 32832268

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showed a trendno p-value reported

However, in piceifrons , several genes showed a trend towards differential expression between the two density conditions.

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an interesting trendno p-value reported

In doing so, we observed an interesting trend when comparing primer efficiencies between different species.

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a clear trendno p-value reported

Likewise, even though NormFinder takes both inter- and intra-group variation into account and should thus perform better under such conditions, this program ranked several genes showing a clear trend towards differential expression between rearing conditions as the most stable in piceifrons .

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