Comparative analysis of restraint stress-induced depressive-like phenotypes in C57BL/6N mice derived from three different sources.

Following RST exposure, C57BL/6NKorl, C57BL/6NA, and C57BL/6NB mice showed a trend towards a reduction in BW, with loss of fat mass implying that reduction of fat mass in body composition is a major factor contributing to the loss of BW caused by repeated RST (Fig. 1 ).

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However, most of these behavioral changes did not reach statistical significance (Fig. 2 ).

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Quantification of TPH-positive cells showed an increasing trend in the dorsal raphé nucleus (DRN, 4.5–4.7 mm posterior from bregma) of C57BL/6NKorl, C57BL/6NA, and C57BL/6NB mice, depending on the RST exposure duration.

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