Electroporation and genetic supply of Cas9 increase the generation efficiency of CRISPR/Cas9 knock-in alleles in C57BL/6J mouse zygotes.

Although individual comparisons at the level of individual gene loci failed to reach statistical significance (Fisher test, p > 0.05 for each gene locus), a marginally significant improvement in overall knock-in efficiency was seen when considering all the gene loci together (Chi squared, p = 0.046, Supplementary Table 6 ), although the large variability in efficiency caused by gene locus complicates this analysis approach (see below for a more rigorous approach).

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3 c) across all three target loci, but again the improvement observed for each gene failed to reach statistical significance (Fisher test, p > 0.05 for each gene locus; see also Supplementary Table 6 ).

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Using this model, electroporation showed a trend towards increased efficiency in overall mutagenesis rate when compared with microinjection, however, this failed to reach statistical significance (β = 0.1448; p value = 0.662).

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