Those CpG sites mapping to Alu sequences showed a highly significant trend of increasing methylation from the older to younger elements ( P <2.2x10 -16 , ANOVA), with mean methylation levels (b ± standard deviation [SD]) of 0.72±0.21 for Alu J, 0.74±0.18 for Alu S, and 0.78±0.17 for Alu Y.
Analysis of subfamilies by age of insertion into the human genome revealed a clear trend of greater hypomethylation of Alu elements in those integrated earliest (Figure 3C; r 2 =0.74, P =0.002).
Among IGHV mutated patients, significant associations with survival were retained for ILRUN and TANK expression (PFDR<0.05), while DCLK2 PFDR=0.0532) approached significance.