Chronic Effects of a High Sucrose Diet on Murine Gastrointestinal Nutrient Sensor Gene and Protein Expression Levels and Lipid Metabolism.

Significant differences in statistic were accepted if p < 0.05, and highly significant differences in statistic were accepted if p < 0.01.

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Serum cholesterol approached significance (1.82 vs. 2.08 mmol/L, p = 0.06, HSD vs. control) while triglycerides (0.71 vs. 0.48 mmol/L; p = 0.7) and NEFA (1.50 vs. 0.85 mmol/L; p = 0.31) were clearly comparable between treatments (HSD vs. control, respectively). 2.2. qPCR Analysis of Small Intestinal Fat Oxidation Genes Expression levels of fat oxidation genes were also studied in the GIT of control fed and HSD mice ( Figure 2 ).

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Ghrl showed a 4-fold induction in the stomach ( Figure 6 a) but did not reach statistical significance ( p = 0.13).

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In HSD mice, gene expression levels of gcg , the precursor of GLP1/2, were downregulated in duodenum and upregulated in ileum, although the fold changes did not quite reach statistical significance.

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