Individual-oocyte transcriptomic analysis shows that genotoxic chemotherapy depletes human primordial follicle reserve in vivo by triggering proapoptotic pathways without growth activation.

Further analysis of the PI3K/PTEN/Akt pathway in the cyclophosphamide-treated samples showed a trend toward a decline in Akt-1 (p = 0.19) and no significant change in the expressions of Foxo3a (p = 0.8) and rpS6 (p = 0.36) (Fig. 3 D–F).

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By qRT-PCR analysis of laser-captured primordial follicle oocyte we observed a trend towards declining expression of Bcl2 (n vehicle = 5 and n chemo = 6; p = 0.17), but together with BAD (n vehicle = 5 and n chemo = 6; p = 0.93), it did not reach statistical significance in the cyclophosphamide -treated xenografts vs. the vehicles (Fig. 5 A,B).

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