The Clinical Value of Measuring Circulating HPV DNA during Chemo-Radiotherapy in Squamous Cell Carcinoma of the Anus.

Fisher’s exact test for difference between all patterns and outcome was highly significant with p < 0.01 ( Figure 3 D).

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The median PT pHPV increased with advancing T stage (T1: 0.35%, T2: 1.26%, T3: 2.29%, T4: 13.46%), but the differences did not reach statistical significance, p = 0.39 ( Figure 2 A) and there was a significantly higher pHPV level in patients with lymph node positive compared to lymph node negative disease, 6.09% (95% CI 2.08–34.54) and 0.39% (95% CI 0.18–2.59), respectively, p = 0.02 ( Figure 2 B).

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Analyses revealed that the pretreatment concentration of pHPV showed a trend towards correlation between stage and level of pHPV, in line with previous observations in other tumor types, and from smaller observations in SCCA [ 18 , 23 ].

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