Depletion of the gut microbiota differentially affects the impact of whey protein on high-fat diet-induced obesity and intestinal permeability.

At 5 weeks, both ABX‐treated groups showed a strong trend toward increased FITC flux compared to non‐treated counterparts (pairwise post‐hoc comparisons not significant due to high between‐animal variability in ABX groups; Figure 5a,b ; Figures S5 and S6 ).

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In addition, HFD‐WPI mice showed a significant increase in liver weight relative to HFD‐CAS mice, whereas HFD‐WPI + ABX mice showed a trend toward an increase in liver weight compared to CAS counterpart, without achieving statistical significance (Figure S1 b).

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