Barely Significant
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Intrinsic motivation for singing in songbirds is enhanced by temporary singing suppression and regulated by dopamine.

Sci Rep · 2021 · PMC8514548 · PMID 34645903

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a strong trendno p-value reported
Nevertheless, there was a strong trend of enhanced singing motivation after long suppression compared to short suppression just like the LO experiments: for individual experimental days, first song latencies tended to be shorter after 5-h SO than after 10-s SO (Fig. 3 C, D top).

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a clear trendno p-value reported
We observed a clear trend that birds sang sooner at higher rates after longer LO durations: first song latencies monotonically decreased as LO duration increased (Fig. 2 B, C; n = 10 birds; p = 0.010, F (3,36) = 4.29, one-way ANOVA; when birds did not produce any songs during the 2-h post-LO period, the data of first song latency was assigned a time of 2 h [120 min]; see Methods ); likewise, initial singing rates monotonically increased as LO durations increased (Fig. 2 B, D; p = 0.0001, F (3,36) = 9.1; when birds did not produce any songs, the data of the initial singing rate was assigned zero).

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