Transsynaptic N-Cadherin Adhesion Complexes Control Presynaptic Vesicle and Bulk Endocytosis at Physiological Temperature.

Acute (5 min) extracellular addition of jasplakinolide (5 μM) did not significantly alter the endocytosis-related SypHy signal decay at autaptic release sites in control cortical neurons, although a strong trend towards an enhancement of vesicle endocytosis was evident ( Figure 6 ).

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Furthermore, a clear trend to an increased amount of endocytosis at 90 s after the end of stimulation was observed in N-cadherin knockout neurons upon acute addition of jasplakinolide ( Figures 6A,B ).

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