As with exogenous synthetic ligand, THP-1 ATP13A1 KO clones infected with E.coli showed a trend of eliciting a lower IFNγ response from sorted human MAIT cells as compared with ATP13A1 sufficient clones ( Figs. 5 B and S14 B ).
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The P5-type ATPase ATP13A1 modulates major histocompatibility complex I-related protein 1 (MR1)-mediated antigen presentation.
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This trend did not reach statistical significance in one repeat due to one sample having high isotype staining ( Fig.