Additionally, when correcting for the cranium centroid size, Thm2 −/− ; Thm1 aln/+ mice showed a significantly wider palatal width compared to the control mice, and the inter-zygomatic arch width approached significant narrowing ( p < 0.053; Figure 4 H,I).
Moreover, when correcting for the cranium centroid size, the Thm2 − / − ; Thm1 aln/+ mice showed smaller Euclidean distance measurements compared to the control mice, including the facial region length, premaxilla length, nasal length, mandibular superior length, mandibular inferior length, upper jaw length, as well as maxillary length, which approached significance ( p < 0.057) ( Figure 4 A–G).
Over 7 days of differentiation, triple allele mutant cells showed a trend toward increased Runx2 ( Figure 6 A).