Overall, amplification of e13a2 was 2% more efficient than e14a2, although this difference did not reach statistical significance (e13a2 median E = 0.972 versus e14a2 = 0.953, P = 0.17, Supplementary Fig. 4A ).
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Impact of BCR::ABL1 transcript type on RT-qPCR amplification performance and molecular response to therapy.
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This difference was statistically significant at the 0.016%, 0.08%, 0.4 and 2% dilution points and approached significance at the 10% dilution.