4E-BP1 levels showed a negative trend during the first 30 min of the treatment and a positive trend within a 60–120 min time frame.
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Ghrelin rapidly elevates protein synthesis in vitro by employing the rpS6K-eEF2K-eEF2 signalling axis.
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At the same time, AMPK pathway, which inhibits translation, showed a trend towards activation, as reported by increased p-AMPKα (T172) and p-ACC (S79) levels (Fig. 2 c, RPPA data).