Assessment of Tissue Specific Distribution and Seasonal Variation of Alkaloids in <i>Alstonia scholaris</i>.

Finally, this work demonstrated that the NMR technique in conjunction with multivariate analysis could play a highly significant role in quality control and herbal extract analysis.

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PC-1 loadings showed a trend in the different parts, moving from negative values to positive values, which indicated the cluster separation due to negative loadings of the Nb-demethylalstogustine N-Oxide and Nb-demethylalstogustine, and positive loadings of picrinine, picralinal, 19 S scholaricine and akuammidine.

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Samples collected on a monthly basis for the study of seasonal variation also showed a significant trend in alkaloidal pattern in PCA and PLS-DA.

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