Sperm quality of artificially matured shortfinned eel is not affected by human chorionic gonadotropin dose and route of administration.

Sperm concentration Throughout the experiment, sperm concentration remained relatively stable at 8–12 ×10 9 ml −1 in all treatment groups, although a near-significant main effect of injection route was evident at Week 10 ( F 1,23 = 4.136, P = 0.054; Fig. 1C ); thus, independent of dose, fish injected IP (Week 10: 11.48 ± 0.50 ×10 9 ml −1 ) produced sperm with a spermatozoa concentration around 15% higher than that observed in fish injected in the muscle (Week 10: 10.19 ± 0.43 ×10 9 ml −1 ).

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Only during Week 10 was a strong trend ( P route = 0.059) detected, relative milt volume (roughly 1.5% of body weight) in IM-injected eels being almost double that of IP-injected eels; injection dose, on the other hand, did not have any effect ( P dose = 0.256).

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