Asymptomatic sensitization to a cow's milk protein induces sustained neuroinflammation and behavioral changes with chronic allergen exposure.

In addition to their hesitance to explore the open zones of the EZM, BLG-sensitized mice on the WP diet also showed a tendency for declined spatial memory by performing fewer successful alternations during the CMT, although the difference did not reach statistical significance ( Figure 3F , sham/WP: 33 ± 2%, n = 12; BLG/WP: 28 ± 2%, n = 12; p = 0.0607, two-way ANOVA, Fisher’s LSD).

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Although CD45-IR cells in the brains of BLG-sensitized mice on the CTL diet showed an increasing trend, the difference from the respective sham group did not reach statistical significance ( Figure 6E , sham/CTL: 0.34 ± 0.07%, n = 9; BLG/CTL: 0.6 ± 0.1%, n = 12, p = 0.3413, two-way ANOVA).

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BLG-sensitized mice with CTL diet showed a trend for elevated serum MCPT-1 levels ( Figure 4A ), although the difference from the respective sham mice did not reach statistical significance (sham/CTL: 1,316 ± 92 pg/ml, n = 11; BLG/CTL: 1,947 ± 298 pg/ml, n = 11; p = 0.07, two-way ANOVA).

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