Complement Regulation in Immortalized Fibroblast-like Synoviocytes and Primary Human Endothelial Cells in Response to SARS-CoV-2 Nucleocapsid Protein and Pro-Inflammatory Cytokine TNFα.

A strong and clearly significant decrease in proliferation was only detected when cells were stimulated with 10% DMSO, which is again consistent with previously described toxicity of DMSO, which blocks the cell cycle at the transition from G1 to S phase [ 42 ].

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Immunofluorescence staining with Ki67 showed a trend of higher proliferative activity of HSE in comparison to K4IM, but significantly confirmed the suppressive effect of NP.

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