<i>Culex</i> Mosquito Piwi4 Is Antiviral against Two Negative-Sense RNA Viruses.

Viruses · 2022 · PMC9781653 · PMID 36560761

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showed a trendno p-value reported

Piwi1, Piwi3, and Zuc silencing showed a trend in 24–32 nt read reduction that was not significant, but silencing of Piwi4, Piwi5, Piwi6a, and Piwi6b significantly reduced the overall portion of 24–32 nt reads, explaining the proportional increase in 21 nt reads.

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a clear trendno p-value reported

Since we noticed a clear trend for reduced piRNA counts in the PIWI gene-silenced samples, we summed up counts from 24 to 32 nt reads to determine if the overall reduction of these putative piRNAs was significant ( Figure 6 d).

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