In vivo examination of healthy human skin after short-time treatment with moisturizers using confocal Raman spectroscopy and optical coherence tomography: Preliminary observations.

18 NMF showed a negative trend from baseline to T1, and we observed a positive trend from T1 to T2 (after suspending treatment).

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In fact, we demonstrated that the thickness of SC did not change significantly at the different time points analyzed, but showed a trend toward reduction at T1 in the treatment arm, which is against the common belief the moisturizers accumulate in and causes the swelling of SC. 36 , 37 Such a reduction of SC thickness after short‐term treatment was also observed in another study with CRS. 19 This might be due to the increased exfoliation of the superficial SC produced by the mechanical action of the regular moisturizer application.

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Parallel to the ET, a positive trend of the water content in the treatment arm could be measured by CRS.

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