Barely Significant
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extremely significant

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extremely significantp<0.051.0× alphaqualified
Except for the control of individual traits, the phenotypic differences of all traits were extremely significant or significant (p<0.05) when considering the single year and two-year averages, and the frequency distribution of all traits was consistent with a normal distribution ( Supplementary Figure S2 ).
extremely significantp -value ≤ 0.051.0× alphaqualified
The expression difference level was defined using the following rules: log2fold change ≥ 0.5 or log2fold change ≤−0.5) and adjusted p -value ≤ 0.01, extremely significant (**); log2fold change ≥ 0.5 or log2fold change ≤−0.5) and 0.01 < adjusted p -value ≤ 0.05, significant (*); otherwise, insignificant. 4.5.
extremely significantp < .051.0× alphaqualified
There existed extremely significant difference between the ACE inhibition value of sample and control within 30 min ( p < .05), which demonstrated that after the pepsin treatment of squash seed protein released bioactive peptides from the native protein structure, and these peptides possessed the ACE inhibitory activity (Figure 5 ). 4.
extremely significantP < 0.051.0× alphaqualified
After 30 days, we observed that mice in the stress group had fewer horizontal movements ( P = 0.0482), fewer vertical movements ( P = 0.0424), and less exercise time ( P = 0.0317) in the OFT and had more immobile times in the FST ( P = 0.0001) and TST ( P = 0.0310), with significant differences or extremely significant differences (* P < 0.05, *** P < 0.001 Student’s t test) when compared with the unstressed group (Figure S 2 A-C).