Longitudinal assessment of fluorescence stability shows fluorescence intensity decreases over time: implications for fluorescence microscopy studies.

PLoS One · 2026 · PMC12962454 · PMID 41785250

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hedged sentences

0.0560

closest p · 1.1× alpha

0.0830

boldest claim

The sentences

a strong trendp = 0.056so close (0.05 < p ≤ 0.1)

Cortical sections did not show a significant decrease in fluorescence intensity, however there was a strong trend towards a decrease ( Fig 8a - 8d ; p = 0.056).

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showed a trendp = 0.083so close (0.05 < p ≤ 0.1)

In contrast, click chemistry staining for EdU (sulfo-Cyanine5 fluorophore), which birthdates cells born at the time of injection, showed a trend towards a decrease in fluorescence intensity using widefield imaging ( Fig 3i - 3l ; p = 0.083) that did not reach significance.

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did not reach statistical significanceno p-value reported

There was a significant decrease in fluorescence intensity for sections stained with the chicken anti-TH antibody ( Fig 7a - 7d ; p = 0.001) but only a trend towards a decrease for the rabbit anti-TH antibody ( Fig 7e - 7h ; p = 0.069) that did not reach statistical significance.

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